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written by
Michael Davidson, Mortimer Abramowitz, and Daniel Axelrod
published by the Olympus America, Inc. and the National High Magnetic Field Laboratory
This item is a comprehensive tutorial on TIRFM (total internal reflection fluorescence microscopy). The technology, developed in the 1980's, is used extensively in cell and molecular biology for observation of thin regions of a specimen, often less than 200nm. This tutorial includes fundamental concepts, theoretical aspects, basic and advanced microscope configurations, and a set of Java applets that depict the fluorophore excitation process and instrument configurations.
Please note that this resource requires Java.
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Record Link
<a href="https://www.compadre.org/portal/items/detail.cfm?ID=6724">Davidson, M, M. Abramowitz, and D. Axelrod. Molecular Expressions: Total Internal Reflection Fluorescence Microscopy. Center Valley: Olympus America, Inc., July 26, 2005.</a>
AIP Format
M. Davidson, M. Abramowitz, and D. Axelrod, (Olympus America, Inc., Center Valley, 1998), WWW Document, (https://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html).
AJP/PRST-PER
M. Davidson, M. Abramowitz, and D. Axelrod, Molecular Expressions: Total Internal Reflection Fluorescence Microscopy (Olympus America, Inc., Center Valley, 1998), <https://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html>.
APA Format
Davidson, M., Abramowitz, M., & Axelrod, D. (2005, July 26). Molecular Expressions: Total Internal Reflection Fluorescence Microscopy. Retrieved May 6, 2025, from Olympus America, Inc.: https://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html
Chicago Format
Davidson, M, M. Abramowitz, and D. Axelrod. Molecular Expressions: Total Internal Reflection Fluorescence Microscopy. Center Valley: Olympus America, Inc., July 26, 2005. https://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html (accessed 6 May 2025).
MLA Format
Davidson, Michael, Mortimer Abramowitz, and Daniel Axelrod. Molecular Expressions: Total Internal Reflection Fluorescence Microscopy. Center Valley: Olympus America, Inc., 1998. 26 July 2005. 6 May 2025 <https://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html>.
BibTeX Export Format
@misc{
Author = "Michael Davidson and Mortimer Abramowitz and Daniel Axelrod",
Title = {Molecular Expressions: Total Internal Reflection Fluorescence Microscopy},
Publisher = {Olympus America, Inc.},
Volume = {2025},
Number = {6 May 2025},
Month = {July 26, 2005},
Year = {1998}
}
Refer Export Format
%A Michael Davidson %A Mortimer Abramowitz %A Daniel Axelrod %T Molecular Expressions: Total Internal Reflection Fluorescence Microscopy %D July 26, 2005 %I Olympus America, Inc. %C Center Valley %U https://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html %O text/html
EndNote Export Format
%0 Electronic Source %A Davidson, Michael %A Abramowitz, Mortimer %A Axelrod, Daniel %D July 26, 2005 %T Molecular Expressions: Total Internal Reflection Fluorescence Microscopy %I Olympus America, Inc. %V 2025 %N 6 May 2025 %8 July 26, 2005 %9 text/html %U https://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html Disclaimer: ComPADRE offers citation styles as a guide only. We cannot offer interpretations about citations as this is an automated procedure. Please refer to the style manuals in the Citation Source Information area for clarifications.
Citation Source Information
The AIP Style presented is based on information from the AIP Style Manual. The APA Style presented is based on information from APA Style.org: Electronic References. The Chicago Style presented is based on information from Examples of Chicago-Style Documentation. The MLA Style presented is based on information from the MLA FAQ. Molecular Expressions: Total Internal Reflection Fluorescence Microscopy:
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Is Part Of
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