Fluorescence Correlation Spectroscopy
published by
the University of Florida
This manual describes an advanced undergraduate laboratory experiment in fluorescence correlation spectroscopy. The experiment uses a single green laser with avalanche photodiode detectors and a software autocorrelator to study the diffusion of fluorescent spheres as well as fluorescently-labeled DNA oligomers. By analysis of the autocorrelation function students can determine the diffusion coefficient of the particles and observe dynamics in the DNA oligomer.
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![]() @misc{
Title = {Fluorescence Correlation Spectroscopy},
Publisher = {University of Florida},
Volume = {2025},
Number = {1 May 2025},
Month = {March 27, 2016},
Year = {2015}
}
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