Fluorescence Correlation Spectroscopy
published by
the Simon Fraser University Department of Physics
Designed for a fourth-year biological physics laboratory, Phys 433, at Simon Fraser University. Contact Nancy Forde (nforde@sfu.ca) or John Bechhoefer (johnb@sfu.ca) for further information.
OBJECTIVES • Learn the physics principles behind fluorescence correlation spectroscopy • Understand the concept of a correlation function • Use the signal detected from FCS of standard-sized particles to determine the extent of the focal volume of a laser beam • Determine the expected FCS signal for particles of different size
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![]() <a href="https://www.compadre.org/portal/items/detail.cfm?ID=13734">Simon Fraser University Department of Physics. Fluorescence Correlation Spectroscopy. Burnaby, British Columbia: Simon Fraser University Department of Physics, August 25, 2015.</a>
![]() (Simon Fraser University Department of Physics, Burnaby, British Columbia, 2014), WWW Document, (https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13734&DocID=4160).
![]() Fluorescence Correlation Spectroscopy (Simon Fraser University Department of Physics, Burnaby, British Columbia, 2014), <https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13734&DocID=4160>.
![]() Fluorescence Correlation Spectroscopy. (2015, August 25). Retrieved May 1, 2025, from Simon Fraser University Department of Physics: https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13734&DocID=4160
![]() Simon Fraser University Department of Physics. Fluorescence Correlation Spectroscopy. Burnaby, British Columbia: Simon Fraser University Department of Physics, August 25, 2015. https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13734&DocID=4160 (accessed 1 May 2025).
![]() Fluorescence Correlation Spectroscopy. Burnaby, British Columbia: Simon Fraser University Department of Physics, 2014. 25 Aug. 2015. 1 May 2025 <https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13734&DocID=4160>.
![]() @misc{
Title = {Fluorescence Correlation Spectroscopy},
Publisher = {Simon Fraser University Department of Physics},
Volume = {2025},
Number = {1 May 2025},
Month = {August 25, 2015},
Year = {2014}
}
![]() %T Fluorescence Correlation Spectroscopy %D August 25, 2015 %I Simon Fraser University Department of Physics %C Burnaby, British Columbia %U https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13734&DocID=4160 %O application/pdf ![]() %0 Electronic Source %D August 25, 2015 %T Fluorescence Correlation Spectroscopy %I Simon Fraser University Department of Physics %V 2025 %N 1 May 2025 %8 August 25, 2015 %9 application/pdf %U https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13734&DocID=4160 Disclaimer: ComPADRE offers citation styles as a guide only. We cannot offer interpretations about citations as this is an automated procedure. Please refer to the style manuals in the Citation Source Information area for clarifications.
Citation Source Information
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