published by the Simon Fraser University Department of Physics

Designed for a fourth-year biological physics laboratory, Phys 433, at Simon Fraser University.  Contact Nancy Forde (nforde@sfu.ca) or John Bechhoefer (johnb@sfu.ca) for further information.

OBJECTIVES
• Learn the physics behind how an optical trap works
• Apply different techniques to determine the stiffness of an optical trap
• Learn about how thermal fluctuations impact a bead's motion, and how an optical
trap attenuates the bead's motion at certain frequencies
• Determine how experimental variables can be used to change the stiffness of an optical
trap
• Learn how power spectral analysis can be used to determine the rotation frequency of
E. coli

• Download trapping.pdf - 2806kb Adobe PDF Document

Last Modified August 25, 2015

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