published by
the Simon Fraser University Department of Physics
Designed for a fourth-year biological physics laboratory, Phys 433, at Simon Fraser University. Contact Nancy Forde (nforde@sfu.ca) or John Bechhoefer (johnb@sfu.ca) for further information.
OBJECTIVES
• Learn the physics principles behind fluorescence correlation spectroscopy
• Understand the concept of a correlation function
• Use the signal detected from FCS of standard-sized particles to determine the extent
of the focal volume of a laser beam
• Determine the expected FCS signal for particles of different size
Last Modified August 25, 2015