DNA electrophoresis Documents

Main Document

DNA electrophoresis 

published by the Simon Fraser University Department of Physics

Designed for a fourth-year (senior) biological physics laboratory, Phys 433, at Simon Fraser University.  Contact Nancy Forde (nforde@sfu.ca) or John Bechhoefer (johnb@sfu.ca) for further information.

OBJECTIVES
• Determine how DNA responds to an electric field and moves through a gel
• Learn about different topological forms of DNA
• Learn how to purify plasmid DNA from E. coli and digest it at a specific site
• Determine a quantitative relationship between the length of DNA and its mobility in a gel

Last Modified August 25, 2015