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written by Michael Davidson, Thomas J. Fellers, Mortimer Abramowitz, and Kenneth R. Spring
published by the Olympus America, Inc. and the National High Magnetic Field Laboratory
This item is a tutorial on the topic of oblique illumination in optical microscopy.  Specimens that are nearly transparent may be almost invisible when viewed in traditional modes.  If illumination is directed to strike the specimen from an oblique angle, details may be revealed with far greater contrast and clarity.  The tutorial includes links to four related Java simulations.  

This item is part of a larger collection of materials on optics and microscopy developed by the National High Magnetic Field Laboratory and Florida State University.

Please note that this resource requires Java.
Subjects Levels Resource Types
General Physics
- Equipment
= Electronic Equipment
Optics
- Geometrical Optics
= Optical Instruments
- Lower Undergraduate
- Upper Undergraduate
- Instructional Material
= Interactive Simulation
= Tutorial
Intended Users Formats Ratings
- Learners
- text/html
- application/java
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Access Rights:
Free access
Restriction:
© 2002 National High Magnetic Field Laboratory
Keywords:
Simulations, anaxial illumination, digital imaging, digital microscopy, inverted digital microscope, microscopy, oblique illumination, off-axis illumination, optics, tutorial
Record Cloner:
Metadata instance created May 12, 2008 by Caroline Hall
ComPADRE is beta testing Citation Styles!

Record Link
AIP Format
M. Davidson, T. Fellers, M. Abramowitz, and K. Spring, (Olympus America, Inc., Center Valley, 2002), WWW Document, (https://micro.magnet.fsu.edu/primer/techniques/oblique/obliquehome.html).
AJP/PRST-PER
M. Davidson, T. Fellers, M. Abramowitz, and K. Spring, Molecular Expressions: Optical Microscopy Primer - Oblique or Anaxial Illumination (Olympus America, Inc., Center Valley, 2002), <https://micro.magnet.fsu.edu/primer/techniques/oblique/obliquehome.html>.
APA Format
Davidson, M., Fellers, T., Abramowitz, M., & Spring, K. (2003, August 1). Molecular Expressions: Optical Microscopy Primer - Oblique or Anaxial Illumination. Retrieved December 12, 2024, from Olympus America, Inc.: https://micro.magnet.fsu.edu/primer/techniques/oblique/obliquehome.html
Chicago Format
Davidson, M, T. Fellers, M. Abramowitz, and K. Spring. Molecular Expressions: Optical Microscopy Primer - Oblique or Anaxial Illumination. Center Valley: Olympus America, Inc., August 1, 2003. https://micro.magnet.fsu.edu/primer/techniques/oblique/obliquehome.html (accessed 12 December 2024).
MLA Format
Davidson, Michael, Thomas J. Fellers, Mortimer Abramowitz, and Kenneth R. Spring. Molecular Expressions: Optical Microscopy Primer - Oblique or Anaxial Illumination. Center Valley: Olympus America, Inc., 2002. 1 Aug. 2003. 12 Dec. 2024 <https://micro.magnet.fsu.edu/primer/techniques/oblique/obliquehome.html>.
BibTeX Export Format
@misc{ Author = "Michael Davidson and Thomas J. Fellers and Mortimer Abramowitz and Kenneth R. Spring", Title = {Molecular Expressions: Optical Microscopy Primer - Oblique or Anaxial Illumination}, Publisher = {Olympus America, Inc.}, Volume = {2024}, Number = {12 December 2024}, Month = {August 1, 2003}, Year = {2002} }
Refer Export Format

%A Michael Davidson %A Thomas J. Fellers %A Mortimer Abramowitz %A Kenneth R. Spring %T Molecular Expressions: Optical Microscopy Primer - Oblique or Anaxial Illumination %D August 1, 2003 %I Olympus America, Inc. %C Center Valley %U https://micro.magnet.fsu.edu/primer/techniques/oblique/obliquehome.html %O text/html

EndNote Export Format

%0 Electronic Source %A Davidson, Michael %A Fellers, Thomas J. %A Abramowitz, Mortimer %A Spring, Kenneth R. %D August 1, 2003 %T Molecular Expressions: Optical Microscopy Primer - Oblique or Anaxial Illumination %I Olympus America, Inc. %V 2024 %N 12 December 2024 %8 August 1, 2003 %9 text/html %U https://micro.magnet.fsu.edu/primer/techniques/oblique/obliquehome.html


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Molecular Expressions: Optical Microscopy Primer - Oblique or Anaxial Illumination:

Contains Molecular Expressions: Optical Microscopy Primer - Oblique Illumination Light Pathway

This is an interactive Java simulation that explores changes in light paths when the illumination angle is changed in an optical microscope.

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