written by
Michael Davidson, Daniel Axelrod, and John Long
published by
the Olympus America, Inc. and the National High Magnetic Field Laboratory
This item is an interactive Java simulation relating to TIRF (total internal reflection fluorescence microscopy). It depicts a configuration known as numerical aperture immersion, which is used in place of a prism to produce excitation at supercritical angles. Users may vary the numerical aperture to increase or decrease incident angles.
This item is part of a larger collection of resources on optics and microscopy developed by the National High Magnetic Laboratory at Florida State University. See Related items below for a link to the full tutorial on total internal reflection fluorescence microscopy.
<a href="https://www.compadre.org/introphys/items/detail.cfm?ID=6732">Davidson, M, D. Axelrod, and J. Long. Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives. Center Valley: Olympus America, Inc., June 15, 2006.</a>
M. Davidson, D. Axelrod, and J. Long, (Olympus America, Inc., Center Valley, 1998), WWW Document, (https://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html).
M. Davidson, D. Axelrod, and J. Long, Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives (Olympus America, Inc., Center Valley, 1998), <https://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html>.
Davidson, M., Axelrod, D., & Long, J. (2006, June 15). Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives. Retrieved March 25, 2025, from Olympus America, Inc.: https://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html
Davidson, M, D. Axelrod, and J. Long. Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives. Center Valley: Olympus America, Inc., June 15, 2006. https://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html (accessed 25 March 2025).
Davidson, Michael, Daniel Axelrod, and John Long. Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives. Center Valley: Olympus America, Inc., 1998. 15 June 2006. 25 Mar. 2025 <https://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html>.
@misc{
Author = "Michael Davidson and Daniel Axelrod and John Long",
Title = {Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives},
Publisher = {Olympus America, Inc.},
Volume = {2025},
Number = {25 March 2025},
Month = {June 15, 2006},
Year = {1998}
}
%A Michael Davidson %A Daniel Axelrod %A John Long %T Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives %D June 15, 2006 %I Olympus America, Inc. %C Center Valley %U https://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html %O application/java
%0 Electronic Source %A Davidson, Michael %A Axelrod, Daniel %A Long, John %D June 15, 2006 %T Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives %I Olympus America, Inc. %V 2025 %N 25 March 2025 %8 June 15, 2006 %9 application/java %U https://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html
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This is the main index for a full set of tutorials by the same authors related to TIRF (total internal reflection fluorescence microscopy). It includes fundamental concepts, theory, microscope configurations, and interactive Java simulations.
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