written by
Michael Davidson, Daniel Axelrod, and John Long
published by
the Olympus America, Inc. and the National High Magnetic Field Laboratory
This item is an interactive Java simulation relating to TIRF (total internal reflection fluorescence microscopy). It depicts the penetration depth of an evanescent field in which waves are propagated to excite fluorophores near the surface interface. The user can manipulate the incident angle and wavelength, and select from a variety of refractive/reflective indices.
This simulation is part of a larger collection of resources on optics and microscopy by the National High Magnetic Field Laboratory at Florida State University. See Related items on this page for a link to the full tutorial on TIRF.
<a href="https://www.compadre.org/introphys/items/detail.cfm?ID=6727">Davidson, M, D. Axelrod, and J. Long. Molecular Expressions: Interactive Java Tutorials - Evanescent Field Penetration Depth. Center Valley: Olympus America, Inc., June 15, 2006.</a>
M. Davidson, D. Axelrod, and J. Long, (Olympus America, Inc., Center Valley, 1998), WWW Document, (https://micro.magnet.fsu.edu/primer/java/tirf/penetration/index.html).
M. Davidson, D. Axelrod, and J. Long, Molecular Expressions: Interactive Java Tutorials - Evanescent Field Penetration Depth (Olympus America, Inc., Center Valley, 1998), <https://micro.magnet.fsu.edu/primer/java/tirf/penetration/index.html>.
Davidson, M., Axelrod, D., & Long, J. (2006, June 15). Molecular Expressions: Interactive Java Tutorials - Evanescent Field Penetration Depth. Retrieved March 16, 2025, from Olympus America, Inc.: https://micro.magnet.fsu.edu/primer/java/tirf/penetration/index.html
Davidson, M, D. Axelrod, and J. Long. Molecular Expressions: Interactive Java Tutorials - Evanescent Field Penetration Depth. Center Valley: Olympus America, Inc., June 15, 2006. https://micro.magnet.fsu.edu/primer/java/tirf/penetration/index.html (accessed 16 March 2025).
Davidson, Michael, Daniel Axelrod, and John Long. Molecular Expressions: Interactive Java Tutorials - Evanescent Field Penetration Depth. Center Valley: Olympus America, Inc., 1998. 15 June 2006. 16 Mar. 2025 <https://micro.magnet.fsu.edu/primer/java/tirf/penetration/index.html>.
@misc{
Author = "Michael Davidson and Daniel Axelrod and John Long",
Title = {Molecular Expressions: Interactive Java Tutorials - Evanescent Field Penetration Depth},
Publisher = {Olympus America, Inc.},
Volume = {2025},
Number = {16 March 2025},
Month = {June 15, 2006},
Year = {1998}
}
%A Michael Davidson %A Daniel Axelrod %A John Long %T Molecular Expressions: Interactive Java Tutorials - Evanescent Field Penetration Depth %D June 15, 2006 %I Olympus America, Inc. %C Center Valley %U https://micro.magnet.fsu.edu/primer/java/tirf/penetration/index.html %O application/java
%0 Electronic Source %A Davidson, Michael %A Axelrod, Daniel %A Long, John %D June 15, 2006 %T Molecular Expressions: Interactive Java Tutorials - Evanescent Field Penetration Depth %I Olympus America, Inc. %V 2025 %N 16 March 2025 %8 June 15, 2006 %9 application/java %U https://micro.magnet.fsu.edu/primer/java/tirf/penetration/index.html
Disclaimer: ComPADRE offers citation styles as a guide only. We cannot offer interpretations about citations as this is an automated procedure. Please refer to the style manuals in the Citation Source Information area for clarifications.
This is the larger collection of materials on TIRF (total internal reflection fluorecence). It contains information on fundamental concepts and theory, as well as multiple java simulations.
Record Link
Save to my folders