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written by Michael Davidson and Mortimer Abramowitz
published by the Olympus America, Inc. and the National High Magnetic Field Laboratory
This item is a comprehensive tutorial on fluorescence microscopy appropriate for science majors.  It includes fundamental concepts, practical applications of the technology, electronic imaging detection, and information about advanced techniques including LSCM, FRET, TIRF, live-cell imaging, confocal microscopy, and contrast combination.  Java simulations and reference materials are also provided.

Please note that this resource requires Java.
Subjects Levels Resource Types
Optics
- Geometrical Optics
= Optical Instruments
= Total Internal Reflection
- Lower Undergraduate
- Instructional Material
= Interactive Simulation
= Tutorial
- Reference Material
Intended Users Formats Ratings
- Educators
- Learners
- text/html
- application/java
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Access Rights:
Free access
Restriction:
© 1998 National High Magnetic Field Laboratory-2008
Keywords:
Simulations, fluorescence microscopy, microscopy, optics, tutorials, virtual microscopes
Record Cloner:
Metadata instance created March 26, 2008 by Caroline Hall
ComPADRE is beta testing Citation Styles!

Record Link
AIP Format
M. Davidson and M. Abramowitz, (Olympus America, Inc., Center Valley, 1998), WWW Document, (https://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html).
AJP/PRST-PER
M. Davidson and M. Abramowitz, Molecular Expressions: Optical Microscopy Primer - Fluorescence Microscopy (Olympus America, Inc., Center Valley, 1998), <https://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html>.
APA Format
Davidson, M., & Abramowitz, M. (2006, July 19). Molecular Expressions: Optical Microscopy Primer - Fluorescence Microscopy. Retrieved December 7, 2024, from Olympus America, Inc.: https://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html
Chicago Format
Davidson, Michael, and Mortimer Abramowitz. Molecular Expressions: Optical Microscopy Primer - Fluorescence Microscopy. Center Valley: Olympus America, Inc., July 19, 2006. https://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html (accessed 7 December 2024).
MLA Format
Davidson, Michael, and Mortimer Abramowitz. Molecular Expressions: Optical Microscopy Primer - Fluorescence Microscopy. Center Valley: Olympus America, Inc., 1998. 19 July 2006. 7 Dec. 2024 <https://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html>.
BibTeX Export Format
@misc{ Author = "Michael Davidson and Mortimer Abramowitz", Title = {Molecular Expressions: Optical Microscopy Primer - Fluorescence Microscopy}, Publisher = {Olympus America, Inc.}, Volume = {2024}, Number = {7 December 2024}, Month = {July 19, 2006}, Year = {1998} }
Refer Export Format

%A Michael Davidson %A Mortimer Abramowitz %T Molecular Expressions: Optical Microscopy Primer - Fluorescence Microscopy %D July 19, 2006 %I Olympus America, Inc. %C Center Valley %U https://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html %O text/html

EndNote Export Format

%0 Electronic Source %A Davidson, Michael %A Abramowitz, Mortimer %D July 19, 2006 %T Molecular Expressions: Optical Microscopy Primer - Fluorescence Microscopy %I Olympus America, Inc. %V 2024 %N 7 December 2024 %8 July 19, 2006 %9 text/html %U https://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html


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Molecular Expressions: Optical Microscopy Primer - Fluorescence Microscopy:

Contains Molecular Expressions: Total Internal Reflection Fluorescence Microscopy

This is a comprehensive tutorial on TIRF, Total Internal Reflection Fluorescence microscopy, appropriate for science majors or students of introductory optics.

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