This is the full tutorial by the same authors on Fluorescence Microscopy.  It discusses applications of the technology, electronic imaging detection, and information about advanced techniques including LSCM, FRET, TIRF, live-cell imaging, confocal microscopy, and contrast combination.

This item is a java simulation depicting depth penetration in a magnetic field in which evanescent waves are propagated.

This interactive Java simulation explores the relationships among evanescent wave intensity, incident angle, and polarization components of the light beam.

This item is an interactive Java simulation depicting High Numerical Aperture Objectives, an instrument configuration lacking a prism.

This is a java simulation depicting a microscope configuration using a trapezoidal prism, one approach for achieving TIRF (total internal reflection fluorescence).