written by
Michael Davidson and Matthew J. Parry-Hill
published by
the Olympus America, Inc. and the National High Magnetic Field Laboratory
This item is an interactive Java simulation relating to the inverted digital microscope. It takes the user on a virtual exploration of "off-axis" illumination, a feature that allows enhancement of contrast in specimens that would otherwise be nearly invisible. Users may choose from more than 15 specimens, including a canine hookworm, a dinosaur bone, and a sample of human kidney tissue. Each image initiates at brightfield and may be examined at varying degrees of oblique, finally reaching darkfield.
This item is part of a larger collection of materials on optics and microscopy developed by the National High Magnetic Field Laboratory and Florida State University. See Related Materials on this page for a link to the full anatomical tutorial on the MIC-D inverted digital microscope.
<a href="https://www.compadre.org/introphys/items/detail.cfm?ID=7029">Davidson, Michael, and Matthew J. Parry-Hill. Molecular Expressions: Olympus MIC-D - Off-Axis Illumination Transitions. Center Valley: Olympus America, Inc., June 15, 2006.</a>
M. Davidson and M. Parry-Hill, (Olympus America, Inc., Center Valley, 2003), WWW Document, (https://micro.magnet.fsu.edu/primer/java/olympusmicd/obliquetransition/index.html).
M. Davidson and M. Parry-Hill, Molecular Expressions: Olympus MIC-D - Off-Axis Illumination Transitions (Olympus America, Inc., Center Valley, 2003), <https://micro.magnet.fsu.edu/primer/java/olympusmicd/obliquetransition/index.html>.
Davidson, M., & Parry-Hill, M. (2006, June 15). Molecular Expressions: Olympus MIC-D - Off-Axis Illumination Transitions. Retrieved June 19, 2025, from Olympus America, Inc.: https://micro.magnet.fsu.edu/primer/java/olympusmicd/obliquetransition/index.html
Davidson, Michael, and Matthew J. Parry-Hill. Molecular Expressions: Olympus MIC-D - Off-Axis Illumination Transitions. Center Valley: Olympus America, Inc., June 15, 2006. https://micro.magnet.fsu.edu/primer/java/olympusmicd/obliquetransition/index.html (accessed 19 June 2025).
Davidson, Michael, and Matthew J. Parry-Hill. Molecular Expressions: Olympus MIC-D - Off-Axis Illumination Transitions. Center Valley: Olympus America, Inc., 2003. 15 June 2006. 19 June 2025 <https://micro.magnet.fsu.edu/primer/java/olympusmicd/obliquetransition/index.html>.
%A Michael Davidson %A Matthew J. Parry-Hill %T Molecular Expressions: Olympus MIC-D - Off-Axis Illumination Transitions %D June 15, 2006 %I Olympus America, Inc. %C Center Valley %U https://micro.magnet.fsu.edu/primer/java/olympusmicd/obliquetransition/index.html %O application/java
%0 Electronic Source %A Davidson, Michael %A Parry-Hill, Matthew J. %D June 15, 2006 %T Molecular Expressions: Olympus MIC-D - Off-Axis Illumination Transitions %I Olympus America, Inc. %V 2025 %N 19 June 2025 %8 June 15, 2006 %9 application/java %U https://micro.magnet.fsu.edu/primer/java/olympusmicd/obliquetransition/index.html
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This is a detailed tutorial on the MIC-D digital microscope, an inverted-illumination system suitable for use in educational or research settings. It includes an anatomical overview and detailed explanations of brightfield, darkfield, oblique, and polarized illumination techniques.