written by
Michael Davidson, Daniel Axelrod, and John Long
published by
the Olympus America, Inc. and the National High Magnetic Field Laboratory
This item is an interactive Java simulation relating to TIRF (total internal reflection fluorescence microscopy). It allows users to explore how changes in the incident angle affect evanescent wave intensity, as well as the relationship between the electric field vectors of each component of the incident beam.
This item is part of a larger collection of tutorials and curriculum support on TIRF and fluorescence microscopy. See Related items on this page for a link to the full tutorial on total internal reflection fluorescence.
<a href="https://www.compadre.org/introphys/items/detail.cfm?ID=6733">Davidson, M, D. Axelrod, and J. Long. Molecular Expressions: Interactive Java Tutorials - Evanescent Field Polarization and Intensity Profiles. Center Valley: Olympus America, Inc., June 15, 2006.</a>
M. Davidson, D. Axelrod, and J. Long, (Olympus America, Inc., Center Valley, 1998), WWW Document, (https://micro.magnet.fsu.edu/primer/java/tirf/evaintensity/index.html).
M. Davidson, D. Axelrod, and J. Long, Molecular Expressions: Interactive Java Tutorials - Evanescent Field Polarization and Intensity Profiles (Olympus America, Inc., Center Valley, 1998), <https://micro.magnet.fsu.edu/primer/java/tirf/evaintensity/index.html>.
Davidson, M., Axelrod, D., & Long, J. (2006, June 15). Molecular Expressions: Interactive Java Tutorials - Evanescent Field Polarization and Intensity Profiles. Retrieved March 23, 2025, from Olympus America, Inc.: https://micro.magnet.fsu.edu/primer/java/tirf/evaintensity/index.html
Davidson, M, D. Axelrod, and J. Long. Molecular Expressions: Interactive Java Tutorials - Evanescent Field Polarization and Intensity Profiles. Center Valley: Olympus America, Inc., June 15, 2006. https://micro.magnet.fsu.edu/primer/java/tirf/evaintensity/index.html (accessed 23 March 2025).
Davidson, Michael, Daniel Axelrod, and John Long. Molecular Expressions: Interactive Java Tutorials - Evanescent Field Polarization and Intensity Profiles. Center Valley: Olympus America, Inc., 1998. 15 June 2006. 23 Mar. 2025 <https://micro.magnet.fsu.edu/primer/java/tirf/evaintensity/index.html>.
@misc{
Author = "Michael Davidson and Daniel Axelrod and John Long",
Title = {Molecular Expressions: Interactive Java Tutorials - Evanescent Field Polarization and Intensity Profiles},
Publisher = {Olympus America, Inc.},
Volume = {2025},
Number = {23 March 2025},
Month = {June 15, 2006},
Year = {1998}
}
%A Michael Davidson %A Daniel Axelrod %A John Long %T Molecular Expressions: Interactive Java Tutorials - Evanescent Field Polarization and Intensity Profiles %D June 15, 2006 %I Olympus America, Inc. %C Center Valley %U https://micro.magnet.fsu.edu/primer/java/tirf/evaintensity/index.html %O application/java
%0 Electronic Source %A Davidson, Michael %A Axelrod, Daniel %A Long, John %D June 15, 2006 %T Molecular Expressions: Interactive Java Tutorials - Evanescent Field Polarization and Intensity Profiles %I Olympus America, Inc. %V 2025 %N 23 March 2025 %8 June 15, 2006 %9 application/java %U https://micro.magnet.fsu.edu/primer/java/tirf/evaintensity/index.html
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This is the index for the full set of tutorials by the same authors on TIRF (Total Internal Reflection Fluorescence Microscopy). It includes fundamental concepts, theory, microscope configurations, and multiple interactive Java simulations.
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