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Fluorescence Correlation Spectroscopy
published by the The University of Florida
This manual describes an advanced undergraduate laboratory experiment in fluorescence correlation spectroscopy.  The experiment uses a single green laser with avalanche photodiode detectors and a software autocorrelator to study the diffusion of fluorescent spheres as well as fluorescently-labeled DNA oligomers.  By analysis of the autocorrelation function students can determine the diffusion coefficient of the particles and observe dynamics in the DNA oligomer.
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Last Modified: December 28, 2015
Subjects Levels Resource Types
Modern Physics
- Biophysics
Other Sciences
- Life Sciences
Thermo & Stat Mech
- Kinetic and Diffusive Processes
= Correlation Function
= Diffusion
- Upper Undergraduate
- Lower Undergraduate
- Instructional Material
= Instructor Guide/Manual
= Laboratory
= Student Guide
Intended Users Formats Ratings
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- Learners
- application/pdf
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Safety Warnings
Eye Protection Must be Worn   Laser Beam  


Access Rights:
Free access
Restriction:
© 2015 The University of Florida, Gainesville.
Keywords:
DNA, Fluorescence correlation spectroscopy, autocorrelation
Record Creator:
Metadata instance created December 28, 2015 by Stephen Hagen
Record Updated:
January 31, 2016 by Ramon Torres-Isea
Last Update
when Cataloged:
December 28, 2015
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AIP Format
(The University of Florida, Gainesville, 2015), WWW Document, (https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13960&DocID=4348).
AJP/PRST-PER
Fluorescence Correlation Spectroscopy (The University of Florida, Gainesville, 2015), <https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13960&DocID=4348>.
APA Format
Fluorescence Correlation Spectroscopy. (2015, December 28). Retrieved April 20, 2024, from The University of Florida: https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13960&DocID=4348
Chicago Format
The University of Florida. Fluorescence Correlation Spectroscopy. Gainesville: The University of Florida, December 28, 2015. https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13960&DocID=4348 (accessed 20 April 2024).
MLA Format
Fluorescence Correlation Spectroscopy. Gainesville: The University of Florida, 2015. 28 Dec. 2015. 20 Apr. 2024 <https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13960&DocID=4348>.
BibTeX Export Format
@misc{ Title = {Fluorescence Correlation Spectroscopy}, Publisher = {The University of Florida}, Volume = {2024}, Number = {20 April 2024}, Month = {December 28, 2015}, Year = {2015} }
Refer Export Format

%T Fluorescence Correlation Spectroscopy %D December 28, 2015 %I The University of Florida %C Gainesville %U https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13960&DocID=4348 %O application/pdf

EndNote Export Format

%0 Electronic Source %D December 28, 2015 %T Fluorescence Correlation Spectroscopy %I The University of Florida %V 2024 %N 20 April 2024 %8 December 28, 2015 %9 application/pdf %U https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13960&DocID=4348


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