Fluorescence Correlation Spectroscopy Documents

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Fluorescence Correlation Spectroscopy 

published by the University of Florida

This manual describes an advanced undergraduate laboratory experiment in fluorescence correlation spectroscopy.  The experiment uses a single green laser with avalanche photodiode detectors and a software autocorrelator to study the diffusion of fluorescent spheres as well as fluorescently-labeled DNA oligomers.  By analysis of the autocorrelation function students can determine the diffusion coefficient of the particles and observe dynamics in the DNA oligomer.

Last Modified January 6, 2016

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