published by
the University of Florida
This manual describes an advanced undergraduate laboratory experiment in fluorescence correlation spectroscopy. The experiment uses a single green laser with avalanche photodiode detectors and a software autocorrelator to study the diffusion of fluorescent spheres as well as fluorescently-labeled DNA oligomers. By analysis of the autocorrelation function students can determine the diffusion coefficient of the particles and observe dynamics in the DNA oligomer.
Last Modified January 6, 2016
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