published by the Simon Fraser University Department of Physics

Designed for a fourth-year biological physics laboratory, Phys 433, at Simon Fraser University.  Contact Nancy Forde (nforde@sfu.ca) or John Bechhoefer (johnb@sfu.ca) for further information.

OBJECTIVES
• Learn the physics principles behind absorption and fluorescence of visible light
• Learn the physics principles behind FRET and how the technique is applied to biological questions
• Learn the physics principles behind light scattering
• Determine the concentration of an unknown solution of dye molecules
• Determine the how light scattering depends on wavelength
• Determine the kinetic stages of self-assembly of collagen proteins into fibrils

• Download spectroscopy.pdf - 892kb Adobe PDF Document

Last Modified August 25, 2015