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written by Michael Davidson, Mortimer Abramowitz, and Daniel Axelrod
published by the Olympus America, Inc. and the National High Magnetic Field Laboratory
This item is a comprehensive tutorial on TIRFM (total internal reflection fluorescence microscopy).  The technology, developed in the 1980's, is used extensively in cell and molecular biology for observation of thin regions of a specimen, often less than 200nm.  This tutorial includes fundamental concepts, theoretical aspects, basic and advanced microscope configurations, and a set of Java applets that depict the fluorophore excitation process and instrument configurations.  

This item is part of a larger collection of materials on optics and microscopy developed by the National High Magnetic Field Laboratory at Florida State University.   See Related items below for a link to the full tutorial on Fluorescence Microscopy.

Please note that this resource requires Java.
Subjects Levels Resource Types
Optics
- Geometrical Optics
= Optical Instruments
= Total Internal Reflection
- Lower Undergraduate
- Instructional Material
= Curriculum support
= Interactive Simulation
= Tutorial
- Reference Material
Intended Users Formats Ratings
- Learners
- Educators
- text/html
- application/java
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Access Rights:
Free access
Restriction:
© 1998 National High Magnetic Field Laboratory-2008
Keywords:
Simulations, TIRF, evanescent field, fluorescence microscopy, fluorophore, microscopy, optics, total internal reflection fluorescence, tutorials, virtual microscopes
Record Cloner:
Metadata instance created March 26, 2008 by Caroline Hall
Record Updated:
August 23, 2008 by Caroline Hall
Last Update
when Cataloged:
July 26, 2005
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Record Link
AIP Format
M. Davidson, M. Abramowitz, and D. Axelrod, (Olympus America, Inc., Center Valley, 1998), WWW Document, (http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html).
AJP/PRST-PER
M. Davidson, M. Abramowitz, and D. Axelrod, Molecular Expressions: Total Internal Reflection Fluorescence Microscopy (Olympus America, Inc., Center Valley, 1998), <http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html>.
APA Format
Davidson, M., Abramowitz, M., & Axelrod, D. (2005, July 26). Molecular Expressions: Total Internal Reflection Fluorescence Microscopy. Retrieved July 29, 2014, from Olympus America, Inc.: http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html
Chicago Format
Davidson, M, M. Abramowitz, and D. Axelrod. Molecular Expressions: Total Internal Reflection Fluorescence Microscopy. Center Valley: Olympus America, Inc., July 26, 2005. http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html (accessed 29 July 2014).
MLA Format
Davidson, Michael, Mortimer Abramowitz, and Daniel Axelrod. Molecular Expressions: Total Internal Reflection Fluorescence Microscopy. Center Valley: Olympus America, Inc., 1998. 26 July 2005. 29 July 2014 <http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html>.
BibTeX Export Format
@misc{ Author = "Michael Davidson and Mortimer Abramowitz and Daniel Axelrod", Title = {Molecular Expressions: Total Internal Reflection Fluorescence Microscopy}, Publisher = {Olympus America, Inc.}, Volume = {2014}, Number = {29 July 2014}, Month = {July 26, 2005}, Year = {1998} }
Refer Export Format

%A Michael Davidson
%A Mortimer Abramowitz
%A Daniel Axelrod
%T Molecular Expressions: Total Internal Reflection Fluorescence Microscopy
%D July 26, 2005
%I Olympus America, Inc.
%C Center Valley
%U http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html
%O text/html

EndNote Export Format

%0 Electronic Source
%A Davidson, Michael
%A Abramowitz, Mortimer
%A Axelrod, Daniel
%D July 26, 2005
%T Molecular Expressions: Total Internal Reflection Fluorescence Microscopy
%I Olympus America, Inc.
%V 2014
%N 29 July 2014
%8 July 26, 2005
%9 text/html
%U http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfhome.html


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Molecular Expressions: Total Internal Reflection Fluorescence Microscopy:

Is Part Of Molecular Expressions: Optical Microscopy Primer - Fluorescence Microscopy

This is the full tutorial by the same authors on Fluorescence Microscopy.  It discusses applications of the technology, electronic imaging detection, and information about advanced techniques including LSCM, FRET, TIRF, live-cell imaging, confocal microscopy, and contrast combination.

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