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written by Michael Davidson, Daniel Axelrod, and John Long
published by the Olympus America, Inc. and the National High Magnetic Field Laboratory
This item is an interactive Java simulation relating to TIRF (total internal reflection fluorescence microscopy).  It depicts a configuration known as numerical aperture immersion, which is used in place of a prism to produce excitation at supercritical angles.  Users may vary the numerical aperture to increase or decrease incident angles.  

This item is part of a larger collection of resources on optics and microscopy developed by the National High Magnetic Laboratory at Florida State University.  See Related items below for a link to the full tutorial on total internal reflection fluorescence microscopy.

Please note that this resource requires Java.
Subjects Levels Resource Types
Optics
- Geometrical Optics
= Optical Instruments
= Total Internal Reflection
- Lower Undergraduate
- Instructional Material
= Curriculum support
= Interactive Simulation
= Tutorial
- Reference Material
Intended Users Formats Ratings
- Learners
- Educators
- application/java
- text/html
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Access Rights:
Free access
Restriction:
© 1998 National High Magnetic Field Laboratory-2008
Keywords:
Brewster's angle, Simulations, TIRF, fluorescence microscopy, high numerical aperture objectives, microscopy, optics, total internal reflection fluorescence, tutorials, virtual microscopes
Record Cloner:
Metadata instance created March 27, 2008 by Caroline Hall
Record Updated:
March 27, 2008 by Caroline Hall
Last Update
when Cataloged:
June 15, 2006
ComPADRE is beta testing Citation Styles!

Record Link
AIP Format
M. Davidson, D. Axelrod, and J. Long, (Olympus America, Inc., Center Valley, 1998), WWW Document, (http://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html).
AJP/PRST-PER
M. Davidson, D. Axelrod, and J. Long, Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives (Olympus America, Inc., Center Valley, 1998), <http://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html>.
APA Format
Davidson, M., Axelrod, D., & Long, J. (2006, June 15). Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives. Retrieved July 28, 2014, from Olympus America, Inc.: http://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html
Chicago Format
Davidson, M, D. Axelrod, and J. Long. Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives. Center Valley: Olympus America, Inc., June 15, 2006. http://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html (accessed 28 July 2014).
MLA Format
Davidson, Michael, Daniel Axelrod, and John Long. Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives. Center Valley: Olympus America, Inc., 1998. 15 June 2006. 28 July 2014 <http://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html>.
BibTeX Export Format
@misc{ Author = "Michael Davidson and Daniel Axelrod and John Long", Title = {Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives}, Publisher = {Olympus America, Inc.}, Volume = {2014}, Number = {28 July 2014}, Month = {June 15, 2006}, Year = {1998} }
Refer Export Format

%A Michael Davidson
%A Daniel Axelrod
%A John Long
%T Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives
%D June 15, 2006
%I Olympus America, Inc.
%C Center Valley
%U http://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html
%O application/java

EndNote Export Format

%0 Electronic Source
%A Davidson, Michael
%A Axelrod, Daniel
%A Long, John
%D June 15, 2006
%T Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives
%I Olympus America, Inc.
%V 2014
%N 28 July 2014
%8 June 15, 2006
%9 application/java
%U http://micro.magnet.fsu.edu/primer/java/tirf/highnaobjective/index.html


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Citation Source Information

The AIP Style presented is based on information from the AIP Style Manual.

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Molecular Expressions: Interactive Java Tutorials - High Numerical Aperture Objectives:

Is Part Of Molecular Expressions: Total Internal Reflection Fluorescence Microscopy

This is the main index for a full set of tutorials by the same authors related to TIRF (total internal reflection fluorescence microscopy).  It includes fundamental concepts, theory, microscope configurations, and interactive Java simulations.

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